Fluids Barriers CNS (2020) 17:51
(link)
Advancing brain barriers RNA sequencing: guidelines from experimental design to publication
David M. F. Francisco, Luca Marchetti, Sabela Rodríguez‑Lorenzo, Eduardo Frías‑Anaya, Ricardo M. Figueiredo, BtRAIN Network, Peter Winter, Ignacio Andres Romero, Helga E. de Vries, Britta Engelhardt and Rémy Bruggmann
Biochimica et Biophysica Acta (BBA) – Biomembranes, 1861/9, 1579-1591, 2019
Lidocaine turns the surface charge of biological membranes more positive and changes the permeability of blood-brain barrier culture models
Ana R. Santa-Maria, Fruzsina R. Walter, Sándor Valkai, Ana Rita Brás, Mária Mészáros, András Kincses, Adrián Klepe, Diana Gaspar, Miguel A.R.B. Castanho, László Zimányi, András Dér, Mária A. Deli
eLife, 8, 2019
Low wnt/β-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice
Fabienne Benz, Viraya Wichitnaowarat, Martin Lehmann, Raoul FV Germano, Diana Mihova, Jadranka Macas, Ralf H Adams, M Mark Taketo, Karl-Heinz Plate, Sylvaine Guérit, Benoit Vanhollebeke, Stefan Liebner
Circulation Research, 124/4, p511-525, 2019
Fine-Tuning of Sox17 and Canonical Wnt Coordinates the Permeability Properties of the Blood-Brain Barrier
Monica Corada, Fabrizio Orsenigo, Ganesh Parameshwar Bhat, Lei Liu Conze, Ferruccio Breviario, Sara Isabel Cunha, Lena Claesson-Welsh, Galina V. Beznoussenko, Alexander A. Mironov, Marco Bacigaluppi, Gianvito Martino, Mara E. Pitulescu, Ralf H. Adams, Peetra Magnusson, Elisabetta Dejana
Scientific Reports, 9/1, 2019
Claudin-3-deficient C57BL/6J mice display intact brain barriers
Mariana Castro Dias, Caroline Coisne, Ivana Lazarevic, Pascale Baden, Masaki Hata, Noriko Iwamoto, David Miguel Ferreira Francisco, Michael Vanlandewijck, Liqun He, Felix A. Baier, Deborah Stroka, Rémy Bruggmann, Ruth Lyck, Gaby Enzmann, Urban Deutsch, Christer Betsholtz, Mikio Furuse, Shoichiro Tsukita, Britta Engelhardt
Circulation Research, 122/2, p231-245, 2018
VE-Cadherin–Mediated Epigenetic Regulation of Endothelial Gene Expression
Marco F. Morini, Costanza Giampietro, Monica Corada, Federica Pisati, Elisa Lavarone, Sara I. Cunha, Lei L. Conze, Nicola O’Reilly, Dhira Joshi, Svend Kjaer, Roger George, Emma Nye, Anqi Ma, Jian Jin, Richard Mitter, Michela Lupia, Ugo Cavallaro, Diego Pasini, Dinis P. Calado, Elisabetta Dejana, Andrea Taddei
The American Journal of Pathology, 187/8, p1879-1892, 2017
Endothelial-to-Mesenchymal Transition in Bone Marrow and Spleen of Primary Myelofibrosis
Benedetta Gaia Erba, Cristian Gruppi, Monica Corada, Federica Pisati, Vittorio Rosti, Niccolo' Bartalucci, Jean-Luc Villeval, Alessandro Maria Vannucchi, Giovanni Barosi, Alessandra Balduini, Elisabetta Dejana
Nature Communications, 8, p14361, 2017
The molecular basis of endothelial cell plasticity
Elisabetta Dejana, Karen K. Hirschi, Michael Simons
STEM CELLS, 35/5, p1328-1340, 2017
Peg3/PW1 Is a Marker of a Subset of Vessel Associated Endothelial Progenitors
Matteo Malinverno, Monica Corada, Luca Ferrarini, Luigi Formicola, Giovanna Marazzi, David Sassoon, Elisabetta Dejana
J Neuroinflammation. 2019 Nov 21;16(1):232. doi: 10.1186/s12974-019-1626-x
Distinct migratory pattern of naive and effector T cells through the blood-CSF barrier following Echovirus 30 infection.
Wiatr M, Stump-Guthier C, Latorre D, Uhlig S, Weiss C, Ilonen J, Engelhardt B, Ishikawa H, Schwerk C, Schroten H, Tenenbaum T, Rudolph H.
2019
David M. F. Francisco, Luca Marchetti, Sabela Rodríguez-Lorenzo, Eduardo Frias, Ricardo M. Figueiredo and their Pis have teamed up to write guidelines for the experimental desing of analysing brain barriers transcriptomes by RNAseq dand how to best publish these observations.
RNA-Seq in its varied forms has revealed itself has an indispensable tool for analyzing the brain barriers transcriptome. The interdisciplinary groups in BtRAIN have identified several shortcomings of current publications on bulk RNA-Seq and single-cell RNA-Seq of the brain barriers. A manuscript is being assembled in which we aim to provide guidelines from experimental design to interpretation of results that will allow to ensure for accurate data interpretation and reliable replication. This publication is dedicated to bridge the gap between the brain barriers research community and the remaining team necessary for an RNA-Seq project, be it sequencing facility technicians or bioinformaticians.
