Advancing brain barriers RNA sequencing: guidelines from experimental design to publication
Lidocaine turns the surface charge of biological membranes more positive and changes the permeability of blood-brain barrier culture models
Low wnt/β-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice
Fine-Tuning of Sox17 and Canonical Wnt Coordinates the Permeability Properties of the Blood-Brain Barrier
Claudin-3-deficient C57BL/6J mice display intact brain barriers
VE-Cadherin–Mediated Epigenetic Regulation of Endothelial Gene Expression
Endothelial-to-Mesenchymal Transition in Bone Marrow and Spleen of Primary Myelofibrosis
The molecular basis of endothelial cell plasticity
Peg3/PW1 Is a Marker of a Subset of Vessel Associated Endothelial Progenitors
Distinct migratory pattern of naive and effector T cells through the blood-CSF barrier following Echovirus 30 infection.
David M. F. Francisco, Luca Marchetti, Sabela Rodríguez-Lorenzo, Eduardo Frias, Ricardo M. Figueiredo and their Pis have teamed up to write guidelines for the experimental desing of analysing brain barriers transcriptomes by RNAseq dand how to best publish these observations.
RNA-Seq in its varied forms has revealed itself has an indispensable tool for analyzing the brain barriers transcriptome. The interdisciplinary groups in BtRAIN have identified several shortcomings of current publications on bulk RNA-Seq and single-cell RNA-Seq of the brain barriers. A manuscript is being assembled in which we aim to provide guidelines from experimental design to interpretation of results that will allow to ensure for accurate data interpretation and reliable replication. This publication is dedicated to bridge the gap between the brain barriers research community and the remaining team necessary for an RNA-Seq project, be it sequencing facility technicians or bioinformaticians.