Comparison of mouse and zebrafish cerebral endothelial cell transcriptomes and functional investigation of conserved pathways governing brain angiogenesis and BBB formation
© Manu Friederich / Universität Bern

ESR 3 vision / project

Raoul Freitas Vale Germano - Universite Libre de Bruxelles

ESR 3

Comparison of mouse and zebrafish cerebral endothelial cell transcriptomes and functional investigation of conserved pathways governing brain angiogenesis and BBB formation

Project Results

The brain vasculature is vital for adequate functioning of the central nervous system, creating a unique and stable microenvironment for neuron activity by tightly controlling the entry of molecules and cells across the blood-brain barrier (BBB). Despite recent advances in understanding brain angiogenesis and barrier induction, the transcriptional regulation and molecular pathways involved in the various stages of brain vascular development and barrier formation remain to be uncovered. To unravel novel genes and pathways involved in BBB functioning in vivo, we isolated zebrafish BBB endothelial cells and analyzed their genome-wide transcription landscape. To acquire an evolutionary conserved BBB geneset, cross-species bioinformatic comparison of the zebrafish BBB transcriptome to analogous expression datasets from mouse embryos was performed. Candidate genes were selected of which the alleged role in BBB function is under current investigation.

Awards

Fondation Rose et Jean Hoguet, Bourse de Doctorat
Fonds Jean Brachet Formations, Travel Grant

Hosting institution

Universite Libre de Bruxelles, Laboratory of Neurovascular Signaling, Belgium

Supervisor

Benoit Vanhollebeke

Early Stage Researcher

Raoul Freitas Vale Germano