Comparison of mouse and zebrafish cerebral endothelial cell transcriptomes and functional investigation of conserved pathways governing brain angiogenesis and BBB formation
© Manu Friederich / Universität Bern

ESR 3 vision / project

Raoul Freitas Vale Germano - Universite Libre de Bruxelles

ESR 3

Comparison of mouse and zebrafish cerebral endothelial cell transcriptomes and functional investigation of conserved pathways governing brain angiogenesis and BBB formation

Objectives

Employing novel in vivo BBB labelling techniques to profile (RNAseq) microvascular endothelial cell gene expression during development, in adults and in previously generated mutant zebrafish embryos with defective BBB properties. Cross-species bioinformatic comparison of the zebrafish BBB transcriptome to analogous expression datasets from mouse embryos will be performed to identify evolutionary conserved pathways and regulatory networks. Analysis of candidate genes and pathways in zebrafish via loss-of-function approaches.

Expected Results

Uncovering molecular pathways that instruct BBB formation and maintenance in vertebrates.

Hosting institution

Universite Libre de Bruxelles, Laboratory of Neurovascular Signaling, Belgium

Supervisor

Benoit Vanhollebeke

Early Stage Researcher

Raoul Freitas Vale Germano