Brain endothelial mechanisms directing the cellular pathway of T cell migration across the BBB
© Manu Friederich / Universität Bern

ESR 6 vision / project

Luca Marchetti - University of Bern

ESR 8

Brain endothelial mechanisms directing the cellular pathway of T cell migration across the BBB

Objectives

We have shown that differential cytokine stimulation of a mouse in vitro BBB model directs T cells to either transcelluar or paracellular routes of diapedesis in vitro. We aim to identify candidates directing trans- vs. para-cellular T cell diapedesis across the BBB by performing serical block-face electron microscopy of T cells migrating across the TNF-a versus IL-1b stimulated in vitro BBB model and by establishing a transcriptome profile of these respective stimulated BBB models. To investigate the role and therapeutic potential of the defined endothelial targets for the cellular pathway of T cell migration across the mouse BBB by live-cell imaging in vitro and in vivo

Expected Results

Definition of BBB molecules different from ICAM-1 directing transcellular vs paracellular T cell diapedesis across the BBB in vitro and in vivo.

Hosting institution

University of Bern, Theodor Kocher Institute, Switzerland

Main Supervisor

Britta Engelhardt

Early Stage Researcher

Luca Marchetti